Phytic acid quantification
Phytic acid content was measured in accordance with Perera et al.
(2019). Fully expanded leaves were homogenized in 0.66 M HCl with quartz
sand, and phytic acid was extracted by shaking the homogenized solution
at 25 oC for 17 h. The extracts were centrifuged
(19,000×g , 10 min at 4 oC) and the supernatant
was neutralized with 0.75 M NaOH. The phytic acid content was measured
by using the Phytic Acid (Total Phosphorus) Assay Kit (Megazyme
International Ireland Ltd., Ireland).