Lipid hydroperoxide quantification
Lipid hydroperoxide (LOOH) in rice leaf blade was quantified by using Spy-LHP (Dojindo, Japan) (Chan et al., 2012). A leaf blade was homogenized in the extraction buffer [Pottasium-phosphate buffer (pH 7.0). 1 mM EDTA, 12.5% (v/v) Glycerol], and the resulting leaf extract was incubated with 2.7 µM Spy-LHP for 30 min on ice. After centrifugation, the supernatant was monitored for fluorescence emission (RF-1500, Shimazu). The excitation and emission wave lengths were 520 and 540 nm, respectively.