Evaluation of hepatic steatosis
The intracellular accumulation of TGs was measured by the AdipoRed assay
according to the manufacturer’s instructions. Briefly, after cell treatments for 48h, chips were rinsed with PBS and incubated with AdipoRed reagent in PBS at room temperature (RT) for 1h, and mean fluorescence intensity (MFI) of the regions of interest (ROIs) occupied by the cells was measured using a laser scanning confocal microscope (A1R MP on an Eclipse Ti-2 inverted microscope, Nikon, Tokyo, Japan) with automated acquisition/analysis software (NIS Elements AR, Nikon), and plotted as fold increase compared to the control (vehicle only).