Chemicals
Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Euroclone (MI, Italy); L-glutamine, penicillin-streptomycin mixture, and AdipoRed Assay Reagent were purchased from Lonza (East Rutherford, NJ, USA); MEM non-essential amino acid solution M7145, palmitic acid (PA; C16:0), oleic acid (OA; C18:1 cis-9), bovine serum albumin (BSA) Cohn fraction V, Hydroxytyrosol (HT) and Quercetin (Que) were purchased from Merck (Darmstadt, Germany); Fetal Bovine Serum (FBS, Gibco), charcoal-stripped FBS, Hoechst 33342 fluorescent nuclear dye and ethidium homodimer-1 (EthD-1) were purchased from Thermo Fisher Scientific (Waltham, MA, USA); ROS-ID Total ROS/Superoxide Detection Kit (ENZ-51010) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA).