4. Conclusion
The results presented herein showed that the substrate used for microbial growth can affect the determination of the heat capacity as well as enthalpy of biological samples. The determination of such heat capacity of a material as a function of the structure of the material can assist in substrate selection for cyanidation wastewater treatment. The impairment caused by free cyanide also reflected on the heat capacity and enthalpy of dry biomass assessed. The toxicity by free cyanide reduces the biomass molecular degree of freedom hence, the biomass could not store sufficient thermal energy as most energy resources are dedicated for cellular maintenance. The enthalpy of combustion of dried biomass of F. oxysporum is within the range available in literatures. This suggests that the method of biomass preparation and its constituents does not significantly affect the final enthalpy of biomass formation. Since biomass are not completely crystalline substance, the enthalpy derived from the calorimetric measurement can be used to further elucidate the capabilities associated with the novel biocatalyst selection for the bioremediation of cyanidation wastewater.