2.4 General experimental procedure for the Knoevenagel reaction
(1a-k→2a–k)
A 5 mL microcentrifuge tube, was charged with solvent (2 mL), to which
the 1a-k (50 mM) and 1,3-dicarbonyl compound (100 mM) were introduced.
The resulting mixture was stirred for the specified amount of time at 25
ºC. After completion, the product was extracted into EtOAc (4×5 mL), The
combined organic layers were dried over anhydrous
Na2SO4 and evaporated under a vacuum.
The residue was purified by flash column chromatography with petroleum
ether and ethyl acetate to afford the desired products.