Figure Legends
Fig. 1: 2-PE production and conversion yield byusing different screened strains (A), Growth and 2-PE production of strain YLG18 under two synthetic pathways (B), Tolerance of strain YLG18 to exogenous 2-PE (C).
Fig. 2: Optimization of single factor fermentation conditions: carbon sources (A), fermentation temperatures (B), initial glucose concentrations (C), initial L-phe concentrations (D).
Fig. 3: Three dimensional response surface and contour plot showing the effect of temperature, initial glucose concentration and initial L-phe concentration on 2-PE production (g/L): the effect of temperature and initial glucose concentration on 2-PE production (g/L) (A), the effect of temperature and initial L-phe concentration on 2-PE production (g/L) (B), the effect of initial glucose concentration and initial L-phe concentration on 2-PE production (g/L) (C), growth and fermentation profiles of strain YLG18 in mineral salts medium amended with 63.54 g/L of glucose and 10.70 g/L of L-phe with temperature controlled at 24.7℃ (D).
Fig. 4: Biosynthesis of 2-PE with ISPR technology: 2-PE yield using different extractants (A); 2-PE production with different phase-volume ratios (B); Effects of adding time of FAME on the growth of cells (C); Effects of FAME adding time on 2-PE production(D).
Fig. 5: Proposed metabolic pathway for 2-PE production within strain YLG18 and comparison of expression levels of related genes in two different 2-PE synthesis pathways.