Observations
RNA localization images with immunofluorescence are accurately and clearly described. Alternative explanations for probe localization such as RNA-DNA hybrids are properly acknowledged with RNase control experiments. However, in the case of the cenRNA-CENP-B co-IF images, there is no mention or explanation of apparent nonspecific staining that is occurring in the 𝛼-satellite (red) channel, which could make these figures harder to interpret on a first read-through. For readers who are non-experts in IF techniques, descriptions of IF images in Figures 2A and 3B will be easy to follow, however the authors may consider elaborating on what they consider to be a ‘juxtaposed’ localization of cenRNAs and CENP-B in reference to their model, and whether this was a general observation.