Significance
Past work of the field has demonstrated that transcription at
centromeres of various eukaryotes is an important and conserved event
for centromere function and chromosomal integrity. As this topic of
research progresses, it will be important to characterize the molecular
mechanism of cenRNAs, which are not understood completely. This research
lays groundwork for investigating further questions on the molecular
mechanisms of cenRNA-mediated CENP-A loading to centromeres. In
particular, the finding that centromere-localizing cenRNAs are of
heterogeneous length and sequence identity invites one to think whether
they could be involved in important RNA-RNA interactions (eg. RNAi-based
methods) that are important for CENP-A loading.