**Figure 4 - Relevance of generalist or specialist kinases.**
**A** Genetic relevance of generalist and specialist kinases. Number of conditions where the kinase is regulated (Absolute estimated kinase activity > 1.75) for each kinase with more than 10 known substrates against the depletion score from CRISPR essentiality screen \cite{26472758}. A lower depletion scores is indicative of kinases that when knocked-out cause severe fitness defects.
**B** Same number of conditions in which a kinase is regulated against the number of phenotypes shown by the knocked-down kinase (from a compilation of RNAi screens www.genomernai.org).
**C** Same number of conditions in which a kinase is regulated against kinase centrality (betweeness) in signalling network. In the inner panel, a toy example illustrates the relationship between betweenness and the signaling network connectivity. Generalist kinases with more than 10 known substrates tend to have also high betweenness scores (Spearman's \(\rho=0.506\), \(p =9.8\times10^{-3}\)). Kinases without shortest paths going through them were excluded.
