**Figure EV2 - Agreement between immunoflorescence and KSEA activities during hES cell differentiation.**
For each of the 10 kinases under study, the panel of phopsho-specific antibodies report the activity at 0, 30 and 60 minutes after PMA stimulation - left panel. Error bars represent mean ± SD. A minimum of 1000 cells were analyzed in each experimental condition. The KSEA activities are inferred from the MS-quantified substrates in the time intervals 0-30 and 0-60 minutes after PMA stimulation and represented as a fraction of the theoretical limit based on the number of KSEA permutations. Note that the MS-based horizontal bars represent a time interval (i.e 0-30min) as compared to the IHC data measured in each individual timepoint (i.e. 30 min). For both time intervals, the relative position of each of the known substrates within the quantitative phosphoproteomic profile indicate enrichments on upregulated - blue - or down-regulated - red- phosphosites - right panels.
