Objective: Increased pelvic floor muscle tone (IPFMt) is associated with somatic symptoms, in different domains, such as sexual dysfunction, lower urinary tract symptoms, defecation problems, pelvic pain and pelvic organ prolapse. This study aims to ascertain the prevalence of symptoms indicating IPFMt in adolescent women. Design: Cross sectional study. Setting: Online spread questionnaire throughout the Netherlands. Population: Women between 18 and 25 years. Methods: This study included Dutch women aged between 18 and 25 years, who had no history of gynecological, urological, gastrointestinal conditions or pregnancy. Participants were recruited through social media and completed the questionnaire ”Amsterdam Hyperactive Pelvic Floor Scale for Women (AHPFS-W)” online. The AHPFS-W distinguishes six different domains of symptoms (max score 30). Main outcome measures: Clinical AHPFS-W cut-off score: no IPFMt (6.00-10.99), mild IPFMt (11.00-12.99), and moderate to severe IPFMt (13.00-30.00). Results: A total of 1,121 eligible women participated in this study, of whom 485 (43.3%) had no indication of IPFMt, and 299 (26.7%) respectively 337 (30.1%) had a mild indication or moderate to severe indication of IPFMt. The highest AHPFS-W score was achieved in the ”Irritable Bowel Symptoms” domain. Conclusions: Among Dutch women aged 18 to 25 years, almost one third reported moderate to severe complaints on the questionnaire, indicating IPFMt. These findings suggest a high prevalence of symptoms that might be associated with IPFMt among adolescent women, thus stressing the need for awareness and further research to IPFMt in this age group.