Yuhao Dai1,2†, Rebecca Pickering1, †, Lucie Cassarino1,3, Seunghee Han4, and Daniel Conley11Department of Geology, Lund University, Lund, Sweden2Research School of Earth Sciences, Australian National University, ACT, Australia3LEMAR laboratory, Institut Universitaire de la Mer, Université de Bretagne Occidental, Plouzané, 29280, France4School of Environmental Science and Technology, Gwangju Institute of Science and Technology, Gwangju, South KoreaCorresponding authors: Yuhao Dai (yuhao.dai@anu.edu.au), Rebecca Pickering (rebecca.pickering@geo.su.se)†These authors contributed equally to this work.Key Points:New downcore measurements show substantial negative apparent silicon isotope fractionation between authigenic clay and pore fluidsSilicon isotope fractionation between authigenic clays and pore fluids appears to depend on supplies of lithogenic materialsAbstractFormation of authigenic clays at sediment-seawater interface and their subsequent burial has recently been recognized as a key process removing silicon from the ocean. However, the effect of authigenic clay formation on isotopic mass balance of silicon in the ocean has been debated. Here, we constrain the apparent silicon isotope (δ30Si) fractionation associated with authigenic clay formation by measuring δ30Si signatures of co-existing pore fluids and reactive authigenic clays in a South Atlantic sediment core. The δ30Si gradients between reactive authigenic clays and pore fluids vary between -2.9 and -0.4‰, supporting a substantial negative δ30Si fractionation associated authigenic clay formation. The variable apparent δ30Si fractionation may be attributable to kinetic effects dependent on availability of lithogenic materials. Overall, our data show that formation and burial of authigenic clay likely preferentially remove isotopically light silicon from the ocean, with important implications on the isotopic mass balance of the marine silica cycle.