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Full-Field Assessment of Geometry and Collagenous Architecture of Porcine Valve Leaflets via Laser Micrometry and Quantitative Polarized Light Imaging
  • Shreya Sreedhar,
  • Daniel P. Pearce,
  • Colleen M. Witzenburg
Shreya Sreedhar
University of Wisconsin System
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Daniel P. Pearce
University of Wisconsin System
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Colleen M. Witzenburg
University of Wisconsin System

Corresponding Author:witzenburg@wisc.edu

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Abstract

Despite the frequent failure of aortic valves and pediatric usage of pulmonary valves as a replacement, comparative studies on their full-field collagenous architecture and macroscale geometries are limited. We applied laser micrometry and quantitative polarized light imaging (QPLI), a novel technique for assessing collagen fiber organization, to porcine aortic (n = 8) and pulmonary (n = 8) valve leaflets to non-destructively compare thickness and anisotropy. We confirmed (1) light intensity and sample thickness are inversely related; and (2) aortic valve leaflets are thicker with decreased fiber organization when unloaded. To demonstrate the ability of QPLI to capture dynamic collagen fiber alignment, we imaged leaflets during equibiaxial loading. There was an increase in the aortic valve leaflet’s degree of alignment throughout loading, whereas the pulmonary valve leaflet exhibited relatively unchanged alignment. Understanding the full-field organization of a leaflet’s heterogeneous ECM and how it is altered by pathology can inform therapy development.
18 Nov 2024Submitted to Journal of Biophotonics
20 Nov 2024Submission Checks Completed
20 Nov 2024Assigned to Editor
20 Nov 2024Review(s) Completed, Editorial Evaluation Pending
20 Nov 2024Reviewer(s) Assigned