Respiratory specimens collected via nasopharyngeal and throat swabs are the recommended method of choice for the molecular detection of the Influenza A virus. However, they often cause discomfort to patients and require trained healthcare workers. This study aimed to validate a cost-effective nucleic acid extraction-free PCR method using SYBR-Green chemistry with saliva samples to diagnose respiratory illnesses caused by Influenza A viruses. This study enrolled symptomatic pediatric and adult patients with influenza-like illness. A SYBR-Green based nucleic acid amplification test was used with CDC-recommended primers specific for detecting Influenza A viruses. Results from saliva PCR tests were compared with those of standard TaqMan chemistry-based PCR detection from nasopharyngeal swabs. The sensitivities of saliva PCR tests with and without nucleic acid extraction were 83% and 82%, respectively. Both the methods showed a specificity of 98%. Overall, the sensitivity and specificity of saliva as a sample using the developed SYBR-Green-based PCR for Influenza A virus detection were 90% and 98%, respectively. Given the high sensitivity, specificity, positive and negative predictive values, likelihood ratios, agreement percentage, and kappa statistics, this study concluded that saliva is a potential specimen for diagnosing Influenza A virus infection by extraction free nucleic acid amplification testing.