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Evaluation of minimum-to-severe global and macrovesicular steatosis in human liver specimens: a portable ambient light-compatible spectroscopic probe
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  • Hao Guo,
  • Ashley E. Stueck,
  • Jason B. Doppenberg,
  • Yun Suk Chae,
  • Alexey B. Tikhomirov,
  • Haishan Zeng,
  • Marten A. Engelse,
  • Boris L. Gala-Lopez,
  • Anita Mahadevan-Jansen,
  • Ian P.J. Alwayn,
  • Andrea Locke,
  • Kevin Hewitt
Hao Guo
Dalhousie University Department of Physics and Atmospheric Science
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Ashley E. Stueck
Dalhousie University Department of Pathology
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Jason B. Doppenberg
Leids Universitair Medisch Centrum
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Yun Suk Chae
Leids Universitair Medisch Centrum
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Alexey B. Tikhomirov
Dalhousie University Department of Physics and Atmospheric Science
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Haishan Zeng
British Columbia Cancer Agency
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Marten A. Engelse
Leids Universitair Medisch Centrum
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Boris L. Gala-Lopez
Dalhousie University Department of Surgery
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Anita Mahadevan-Jansen
Vanderbilt University
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Ian P.J. Alwayn
Leids Universitair Medisch Centrum
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Andrea Locke
Vanderbilt University
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Kevin Hewitt
Dalhousie University Department of Physics and Atmospheric Science

Corresponding Author:kevin.hewitt@dal.ca

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Abstract

This study presents a portable spectroscopic system compatible with ambient light to assess hepatic steatosis (HS) and macrovesicular steatosis (MaS) in human liver specimens. Traditional assessment methods for MaS are limited, prompting the need for non-invasive alternatives. The study utilized a two-stage approach on thawed snap-frozen liver specimens. Biochemical validation compared fat content from Raman and reflectance intensities with triglyceride (TG) quantifications, while histopathological validation contrasted Raman-derived fat content with pathologist evaluations and an algorithm. Analysis of 16 specimens showed a positive correlation between spectroscopic data and TG quantifications. The Raman system differentiated various degrees of global HS and MaS in an additional 66 specimens. A dual-variable prediction algorithm classified significant discrepancies (≥10%) between algorithm-estimated global HS and pathologist-estimated MaS. This study demonstrates the viability of a portable spectroscopic system for non-invasive HS and MaS assessment to enhance real-time donor liver assessments during recovery to improve transplantation outcomes.
Submitted to Journal of Biophotonics
Assigned to Editor
Submission Checks Completed
Reviewer(s) Assigned
28 Jun 2024Review(s) Completed, Editorial Evaluation Pending
28 Jun 2024Reviewer(s) Assigned
06 Aug 2024Editorial Decision: Revise Major
27 Aug 20241st Revision Received
29 Aug 2024Submission Checks Completed
29 Aug 2024Assigned to Editor
29 Aug 2024Review(s) Completed, Editorial Evaluation Pending
29 Aug 2024Reviewer(s) Assigned
16 Sep 2024Editorial Decision: Revise Minor
20 Sep 20242nd Revision Received
23 Sep 2024Submission Checks Completed
23 Sep 2024Assigned to Editor
23 Sep 2024Reviewer(s) Assigned
23 Sep 2024Review(s) Completed, Editorial Evaluation Pending
23 Sep 2024Editorial Decision: Accept