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A novel MsEOBI-MsPAL1 module enhances salinity stress tolerance, floral scent emission, and seed yield in alfalfa
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  • Yuguang Song,
  • Haoyan Tang,
  • Zhaoran Zhang,
  • Xueying Sun,
  • Xinru Ding,
  • Xinying Guo,
  • Qi Wang,
  • Jifeng Chen,
  • Wei Dong
Yuguang Song
Qufu Normal University
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Haoyan Tang
Qufu Normal University
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Zhaoran Zhang
Qufu Normal University
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Xueying Sun
Qufu Normal University
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Xinru Ding
Qufu Normal University
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Xinying Guo
Qufu Normal University
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Qi Wang
Qufu Normal University
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Jifeng Chen
Qufu Normal University
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Wei Dong
Qufu Normal University

Corresponding Author:dwei@qfnu.edu.cn

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Abstract

Alfalfa ( Medicago sativa L.) is a crucial and widely grown forage legume that faces yield challenges due to salinity stress. In this study, an R2R3-MYB transcription factor (TF), MsEOBI was characterized from alfalfa. Its function and gene regulatory pathways were studied in alfalfa seedlings. The nuclear-localized MsEOBI acts as a transcriptional activator. MsEOBI enhances salinity tolerance of alfalfa by promoting flavonoids and lignin biosynthesis, as well as scavenging reactive oxygen species (ROS). MsEOBI also promote pollinator attraction and seed yield by activating volatile phenylpropanoid biosynthesis. Yeast one-hybrid, dual-luciferase, and ChIP-qPCR assays showed that MsEOBI binds directly to the promoter of MsPAL1, a key enzyme in the phenylpropanoid pathway to activate its expression. Overexpression of MsPAL1 in alfalfa enhances salinity tolerance. These findings shed light on the functions of the MsEOBI-MsPAL1 module in alfalfa and offer valuable genetic resources for the breeding of salt stress-resistant varieties in the future.
Submitted to Plant, Cell & Environment
Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
25 Jun 2024Review(s) Completed, Editorial Evaluation Pending
25 Jun 2024Reviewer(s) Assigned
29 Aug 20241st Revision Received
06 Sep 2024Submission Checks Completed
06 Sep 2024Assigned to Editor
06 Sep 2024Review(s) Completed, Editorial Evaluation Pending
09 Sep 2024Reviewer(s) Assigned
15 Sep 2024Editorial Decision: Accept