Plants are naturally affected against wide range of human diseases. They were used from ancient times for several purposes including cooking, preserving food, for treating a wide range of health conditions including cold, dysentery, anemia, wound heeling, curing insect bites and injuries. Nowadays people prefer products derived from natural sources more then products that are made from chemical methods. The plant Ocimum basilicum has a very range of health benefits including its potency to cure several skin conditions. This study aims to develop ,evaluate ,and determine the effectiveness of the herbal ointment made from Ocimum basilicum without alcohol and bees wax, using chea butter as thickening agent with the main focus on determining its outcome on common dermatological conditions such as acne(bacterial infection),eczema, inflammation, minor cuts, scrape wound. The prepared ointment was then evaluated for physiochemical characteristics including safety assessment of skin and stability tests. Then In Vivo experiments were carried in a group of volunteers including teenage and adults(two boys and three girls) skin, it reduced redness, inflammation and cured acne. The results showed the wound heeling ,anti-inflammatory and anti-bacterial activity of this ointment .In conclusion ,no adverse reaction of this ointment was observed on skin , The thorough evaluation of this ointment has confirmed its anti-bacterial activity because of its ability to kill bacterial strains causing acne. Further research is needed for its commercial usage and clinical trials to use for Post surgery wounds, serious bacterial and fungal infections.