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Aggregation-based Dual-Target Probe for Dual-Color Super-Resolution Monitoring Mitophagy and Evaluating Drugs Regulating Mitochondria
  • +6
  • Xiu-Zhi Yang,
  • Hongbao Fang,
  • Shumeng Li,
  • Chengyan Chu,
  • Yunhua Zhang,
  • Ying Yang,
  • Weijiang He,
  • Yuncong Chen,
  • Zijian Guo
Xiu-Zhi Yang
Nanjing University State Key Laboratory of Coordination Chemistry
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Hongbao Fang
Nanjing Normal University School of Chemistry and Material Science
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Shumeng Li
Nanjing University State Key Laboratory of Coordination Chemistry
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Chengyan Chu
Nanjing University State Key Laboratory of Coordination Chemistry
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Yunhua Zhang
Nanjing University State Key Laboratory of Coordination Chemistry
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Ying Yang
Nanjing University State Key Laboratory of Coordination Chemistry
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Weijiang He
Nanjing University State Key Laboratory of Coordination Chemistry
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Yuncong Chen
Nanjing University State Key Laboratory of Coordination Chemistry

Corresponding Author:chenyc@nju.edu.cn

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Zijian Guo
Nanjing University State Key Laboratory of Coordination Chemistry
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Abstract

Developing single fluorescent probe for simultaneously visualizing mitophagy flux and oxidative stress with super-resolution is highly demanded yet quite challenging. Herein, we design and synthesize a ratiometric HClO probe AHOH, which is capable of simultaneously staining lysosomes and mitochondria with red and green color, respectively. AHOH could be selectivity oxidized by HClO, leading to a large emission blue shift (90 nm) and an over 1300-fold enhancement of the emission ratio of Fl547 nm/Fl637 nm. We apply AHOH in super-resolution microscopy and clearly visualize the dynamics of mitochondria-lysosomes interactions and the oxidative stress states upon different stimuli. Mitochondria dysfunction triggered by different drugs and genetic defect lead to elevated oxidative stress and higher levels of mitophagy. Moreover, AHOH could serve as a reliable tool for evaluating the efficacy of drugs regulating mitochondria dysfunction. This work provides a powerful dual-color super-resolution imaging agent for real time monitoring the dynamics of organelle interactions and oxidative stress.
Submitted to Aggregate
Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
30 Jun 2024Reviewer(s) Assigned
10 Jul 2024Review(s) Completed, Editorial Evaluation Pending
11 Jul 2024Editorial Decision: Revise Minor
20 Jul 20241st Revision Received
22 Jul 2024Submission Checks Completed
22 Jul 2024Assigned to Editor
24 Jul 2024Reviewer(s) Assigned
28 Jul 2024Review(s) Completed, Editorial Evaluation Pending
28 Jul 2024Editorial Decision: Accept