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A Novel Laccase for Alkaline Medium Temperature Environments in the Textile Industry
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  • Kaifeng Xu,
  • Ying Huo,
  • Shiming Tang,
  • Shuangyan Han,
  • Ying Lin ,
  • Suiping Zheng
Kaifeng Xu
South China University of Technology School of Biology and Biological Engineering
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Ying Huo
South China University of Technology School of Biology and Biological Engineering
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Shiming Tang
South China University of Technology School of Biology and Biological Engineering
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Shuangyan Han
South China University of Technology School of Biology and Biological Engineering
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Ying Lin
South China University of Technology School of Biology and Biological Engineering
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Suiping Zheng
South China University of Technology School of Biology and Biological Engineering

Corresponding Author:spzheng@scut.edu.cn

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Abstract

Laccases have a wide range of uses in the textile industry for their ability to decolorize dyes, modify the surface of fabrics and bleach textiles. Screening a laccase with high thermal stability and alkali tolerance suitable for textile applications is challenging. In this study, we identified a novel alkaline laccase, LacCT, from Caldalkalibacillus thermarum, and expressed it in Escherichia coli. It showed an optimal temperature of 65°C and a pH range of high stability from 6.0 to 10.0, with an optimum pH of 7.5. Through rational design, the thermal stability of the best variant, G190P/Q254Y/G336M/D510F (LacCT-11), was significantly improved, exhibiting a half-life of 63.2 minutes at 60°C, 1.8 times longer than the wild type. This research presents a new laccase with significant potential for decolorizing textile wastewater and enhancing the ramie degumming process.
Submitted to Biotechnology Journal
Submission Checks Completed
Assigned to Editor
Reviewer(s) Assigned
25 Jun 2024Review(s) Completed, Editorial Evaluation Pending
11 Jul 2024Editorial Decision: Revise Major
20 Aug 20241st Revision Received
21 Aug 2024Submission Checks Completed
21 Aug 2024Assigned to Editor
21 Aug 2024Review(s) Completed, Editorial Evaluation Pending
23 Aug 2024Editorial Decision: Accept