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Enhanced natural killer cell proliferation by stress-induced feeder cells
  • Donghyun Lee,
  • Myeongkwan Song,
  • Soonjo Kwon
Donghyun Lee
Inha University
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Myeongkwan Song
Inha University
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Soonjo Kwon
Inha University

Corresponding Author:soonjo.kwon@inha.ac.kr

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Abstract

Natural Killer (NK) cells, integral to the innate immune system, are notable in cell therapies because for their applicability in allogeneic treatments, distinguishing them from T cells typically employed in conventional cell therapies. However, their limited half-life poses a challenge for therapy. Although attempts to leverage feeder cells are common, safer methods are needed to mitigate the associated risks. In our study, an upregulation in the expression of 4-1BBL in Colo-205 cells under extracellular stresses such as hypoxia and cytochalasin D was observed. This enhanced binding to the 4-1BB receptors on NK cells promotes proliferation in NK cells. Elevated CD56 expression of a marker strongly linked to NK cell proliferation in co-culture further supports this process. Applying extracellular stressors, specifically hypoxia and cytochalasin D, to Colo-205 cells successfully tailored feeder cells, significantly enhancing NK cell proliferation.
15 Apr 2024Submitted to Biotechnology and Bioengineering
23 Apr 2024Submission Checks Completed
23 Apr 2024Assigned to Editor
23 Apr 2024Review(s) Completed, Editorial Evaluation Pending
13 May 2024Reviewer(s) Assigned
09 Jun 2024Editorial Decision: Revise Major
05 Sep 2024Reviewer(s) Assigned
08 Sep 2024Review(s) Completed, Editorial Evaluation Pending
08 Sep 2024Editorial Decision: Revise Minor
10 Sep 20242nd Revision Received
11 Sep 2024Submission Checks Completed
11 Sep 2024Assigned to Editor
11 Sep 2024Review(s) Completed, Editorial Evaluation Pending