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Comprehensive Characterization of Protein Modifications using Mass Spectrometry and Dry Blood Spots
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  • Sofia Guedes,
  • Luis Perpetuo,
  • Jacinta Jacinta,
  • Tânia Lima,
  • Ines Pires,
  • Francisca Savaiva,
  • André Lourenço,
  • Liliana Moreira,
  • Adelino Moreira,
  • Antonio Barros,
  • Fabio Trindade,
  • Rui Vitorino
Sofia Guedes
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Luis Perpetuo
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Jacinta Jacinta
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Tânia Lima
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Ines Pires
University of Porto Faculty of Medicine
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Francisca Savaiva
University of Porto Faculty of Medicine
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André Lourenço
University of Porto Faculty of Medicine
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Liliana Moreira
University of Porto Faculty of Medicine
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Adelino Moreira
University of Porto Faculty of Medicine
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Antonio Barros
University of Porto Faculty of Medicine
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Fabio Trindade
University of Porto Faculty of Medicine
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Rui Vitorino

Corresponding Author:rvitorino@ua.pt

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Abstract

Abstract Purpose The main objective of this study is to characterize and analyze modified peptides in DBS samples. This includes deciphering their specific PTMs and understanding their potential impact on the population or disease cohort under study. Experimental design Using mass spectrometry-based proteomic approaches, we performed a comprehensive analysis of DBS samples. Our focus was on the identification and quantification of modified peptides. We also took advantage of recent advances in DBS mass spectrometry to ensure accurate detection and quantification. Results A comprehensive analysis identified 976 modified peptides in DBS samples. Of these, a subset of 211 peptides was consistently present in all samples, highlighting their potential biological importance and relevance. This indicates a diverse spectrum of PTMs in the proteome of DBS samples. Conclusions and clinical relevance Integration of mass spectrometry and proteomics has revealed a broad spectrum of modified peptides in DBS samples and highlighted their importance in biological processes and disease progression. Accurate detection of these PTMs may be critical for risk stratification and disease management. This study improves the understanding of molecular mechanisms underlying biological processes and disease development, providing important insights for clinical applications.
22 Aug 2023Submitted to Clinical Applications
31 Aug 2023Submission Checks Completed
31 Aug 2023Assigned to Editor
31 Aug 2023Review(s) Completed, Editorial Evaluation Pending
31 Aug 2023Reviewer(s) Assigned
21 Oct 2023Review(s) Completed, Editorial Evaluation Pending
21 Oct 20231st Revision Received
26 Oct 2023Reviewer(s) Assigned
15 Nov 2023Editorial Decision: Revise Major
19 Nov 2023Review(s) Completed, Editorial Evaluation Pending
19 Nov 20232nd Revision Received
22 Nov 2023Reviewer(s) Assigned