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Role of narL gene in the pathogenesis of Salmonella Typhimurium
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  • PASHUPATHI M,
  • Swagatika Priyadarsini ,
  • Nikhil K C,
  • Pravas Ranjan Sahoo,
  • Rohit Singh,
  • Meeta Saxena,
  • Vikramaditya Upmanyu,
  • Ravikant Agarwal,
  • Praveen Singh,
  • Mohini Saini,
  • AJAY KUMAR
PASHUPATHI M
Indian Veterinary Research Institute
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Swagatika Priyadarsini
College of Veterinary Science and Animal Husbandry
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Nikhil K C
College of Veterinary Science and Animal Husbandry
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Pravas Ranjan Sahoo
Indian Veterinary Research Institute
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Rohit Singh
Indian Veterinary Research Institute
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Meeta Saxena
Indian Veterinary Research Institute
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Vikramaditya Upmanyu
Indian Veterinary Research Institute
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Ravikant Agarwal
Indian Veterinary Research Institute
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Praveen Singh
Indian Veterinary Research Institute
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Mohini Saini
Indian Veterinary Research Institute
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AJAY KUMAR
Indian Veterinary Research Institute

Corresponding Author:ajayivri@gmail.com

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Abstract

Salmonella Typhimurium (STM) is a facultative anaerobe of zoonotic importance and one of the causative agents of non-typhoidal salmonellosis (NTS). During infection, STM must adapt to the changes in oxygen concentration encountered in the crucial niches of host like gut lumen and intramacrophage environments. But being a chemo-organoheterotroph, STM is capable of obtaining its energy from organic sources via redox reactions. NarL, a transcription factor and the response regulator of the two-component regulatory system NarX/L, gets activated under nitrate rich anaerobic condition. Upon activation, it upregulates the nitrate reduction during anaerobic respiration. However, in this study, we observed a significant attenuation of virulence in the narL-knockout strain of STM, while the respective morphotypes got rescued upon genetic complementation. Along with motility and biofilm forming ability, the mutant strain displayed reduced intracellular replication in either intestinal epithelial cells or monocyte-derived macrophages of poultry origin. Further, in vivo competitive assay in the murine model showed that wild type STM significantly outcompeted its isogenic narL null mutant.
07 Aug 2023Submitted to Journal of Basic Microbiology
10 Aug 2023Submission Checks Completed
10 Aug 2023Assigned to Editor
10 Aug 2023Review(s) Completed, Editorial Evaluation Pending
23 Aug 2023Reviewer(s) Assigned
06 Sep 2023Editorial Decision: Revise Major
03 Oct 20231st Revision Received
04 Oct 2023Submission Checks Completed
04 Oct 2023Assigned to Editor
04 Oct 2023Review(s) Completed, Editorial Evaluation Pending
07 Oct 2023Reviewer(s) Assigned
01 Nov 2023Editorial Decision: Accept