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Microscopic morphology and distribution of the antennal sensilla in the double spine bark beetle, Ips duplicatus
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  • Mayuri Kashinath Shewale,
  • Jana Nebesarova,
  • Ewald Grosse- Wilde,
  • Blanka Kalinová
Mayuri Kashinath Shewale
Ceska Zemedelska Univerzita v Praze Fakulta lesnicka a drevarska

Corresponding Author:shewale@fld.czu.cz

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Jana Nebesarova
Univerzita Karlova
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Ewald Grosse- Wilde
Ceska Zemedelska Univerzita v Praze Fakulta lesnicka a drevarska
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Blanka Kalinová
Ceska Zemedelska Univerzita v Praze Fakulta lesnicka a drevarska
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Abstract

ABSTRACT The double-spined spruce bark beetle, Ips duplicatus has become an infamous secondary pest of Norway spruce, causing extensive ecological and economic destruction in many Central European countries. Antennae are the primary olfactory organs that play fundamental role in insect-host chemical communication; therefore, understanding morphology is crucial before conducting electrophysiological investigations. Here, we present our analysis of sensilla types on the antennal surface of I. duplicatus for the first time, using high-resolution-scanning electron microscopy. We studied the external morphological characteristics of antennae and the types, numbers, and distribution of the antennal sensilla in males and females. Our results revealed the presence of five different types of morphologically distinct sensilla: sensilla chaetica, sensilla basiconica, sensilla trichodea, sensilla coeloconica, and Bӧhm’s bristles. We observed two subtypes in sensilla chaetica (SChI and SChII), four subtypes in sensilla basiconica (SBI, SBII, SBIII and SBIV), three subtypes in sensilla trichodea (STrII, STrIII and STrIV) and two subtypes in sensilla coeloconica (SCoI and SCoII), respectively in I. duplicatus males and females. Minor differences in length and numbers between the sexes for some sensilla types were found. Distribution maps for different sensillar types were constructed and specific areas for the respective sensilla were found. Possible functions of observed sensilla types are discussed. The present study provides a basis for future electrophysiological studies aimed at understanding how I. duplicatus detects ecologically important olfactory cues.
03 Jul 2023Submitted to Microscopy Research and Technique
03 Jul 2023Submission Checks Completed
03 Jul 2023Assigned to Editor
10 Jul 2023Review(s) Completed, Editorial Evaluation Pending
10 Jul 2023Reviewer(s) Assigned
17 Jul 2023Editorial Decision: Revise Minor
27 Jul 20231st Revision Received
27 Jul 2023Submission Checks Completed
27 Jul 2023Assigned to Editor
27 Jul 2023Review(s) Completed, Editorial Evaluation Pending
28 Jul 2023Editorial Decision: Accept