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On-line targeted metabolomics for real-time monitoring of relevant compounds in fermentation processes
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  • Joan Cortada Garcia,
  • Jennifer Haggarty,
  • Stefan Weidt,
  • Ronan Daly,
  • Alison Arnold,
  • Karl Burgess
Joan Cortada Garcia
The University of Edinburgh School of Biological Sciences
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Jennifer Haggarty
University of Glasgow
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Stefan Weidt
University of Glasgow
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Ronan Daly
University of Glasgow
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Alison Arnold
Ingenza Ltd
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Karl Burgess
The University of Edinburgh School of Biological Sciences

Corresponding Author:karl.burgess@ed.ac.uk

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Abstract

Fermentation monitoring is a powerful tool for bioprocess development and optimisation. On-line metabolomics is a technology that is starting to gain attention as a bioprocess monitoring tool, allowing the direct measurement of many compounds in the fermentation broth at a very high time resolution. In this work, targeted on-line metabolomics was used to monitor 40 metabolites of interest during three Escherichia coli succinate production fermentation experiments every 5 minutes with a triple quadrupole mass spectrometer. This allowed capturing high time resolution biological data that can provide critical information for process optimisation. For 9 of these metabolites, simple univariate regression models were used to model compound concentration from their on-line mass spectrometry peak area. These on-line metabolomics univariate models performed comparably to vibrational spectroscopy multivariate PLS regressions models reported in the literature, which typically are much more complex and time consuming to build. In conclusion, this work shows how on-line metabolomics can be used to directly monitor many bioprocess compounds of interest and obtain rich biological and bioprocess data.
19 May 2023Submitted to Biotechnology and Bioengineering
26 May 2023Submission Checks Completed
26 May 2023Assigned to Editor
26 May 2023Review(s) Completed, Editorial Evaluation Pending
12 Jul 2023Reviewer(s) Assigned
25 Aug 2023Editorial Decision: Revise Minor
09 Oct 20231st Revision Received
30 Oct 2023Review(s) Completed, Editorial Evaluation Pending
30 Oct 2023Editorial Decision: Accept