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Sperm Morphology and Comparison Morphometry Measurements of two Species of Bats, Molossus molossus and Molossops temminckii (Chiroptera: Molossidae)
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  • ANA SOFÍA DIP,
  • EDUARDO, MARTÍN,
  • RUBÉN ALDO ANDRADA,
  • MARCELA BEATRIZ HERNÁNDEZ,
  • MARÍA DANIELA MIOTTI
ANA SOFÍA DIP
Universidad Nacional de Tucuman Facultad de Ciencias Naturales e Instituto Miguel Lillo

Corresponding Author:anadip1315@gmail.com

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EDUARDO, MARTÍN
Universidad Nacional de Tucuman Facultad de Ciencias Naturales e Instituto Miguel Lillo
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RUBÉN ALDO ANDRADA
Fundacion Miguel Lillo
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MARCELA BEATRIZ HERNÁNDEZ
Fundacion Miguel Lillo
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MARÍA DANIELA MIOTTI
Universidad Nacional de Tucuman Facultad de Ciencias Naturales e Instituto Miguel Lillo
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Abstract

Sperm morphology is considered a species-specific character and has been used as a tool in the classification of numerous mammalian taxa. Neotropical bats have been poorly studied, and important aspects on sperm morphology have not been elucidated. The aim of the present study was to describe and compare the sperm morphology and morphometry of Molossus molossus and Molossops temminckii. 14 adults specimens were analyzed from the Colección Mamíferos Lillo, Universidad Nacional de Tucumán: five Molossus molossus and nine Molossops temminckii. The epididymis were extracted and macerated in Farmer’s solution, followed by a coloration with different stains. To carry out the description and morphometric analysis, microphotographs were taken under an optical, epifluorescence and scanning electron microscope. A total of 50 sperm from each individual were measured for morphometric analysis. The length and width of the head, midpiece and tail were taken as variables. Sperm from M. molossus and M. temminckii were practically identical, both morphologically and morphometrically. In both species, a distal bulge was observed at the end of the intermediate piece in a percentage greater than 85%. The main characteristics shared between the species were: presence of acrosomal blebs in the upper half of the head of the spermatozoa; cephalic equatorial segment with filiform ornamentations; intermembrane space of head apex wedge-shaped; helical middle piece and annulus at the end of middle piece. In the present study, scanning electron microscopy allowed us to visualize structures, such as acrosomal vesicles, that were not detected with other types of microscopy.
30 Mar 2023Submitted to Microscopy Research and Technique
04 Apr 2023Submission Checks Completed
04 Apr 2023Assigned to Editor
25 Apr 2023Review(s) Completed, Editorial Evaluation Pending
25 Apr 2023Reviewer(s) Assigned
26 May 2023Editorial Decision: Revise Major
29 Jun 20231st Revision Received
18 Jul 2023Submission Checks Completed
18 Jul 2023Assigned to Editor
18 Jul 2023Review(s) Completed, Editorial Evaluation Pending
20 Jul 2023Reviewer(s) Assigned
25 Jul 2023Editorial Decision: Accept