loading page

A lentinan-loaded calcium alginate hydrogel with a core-shell structure induces broad-spectrum resistance to plant viruses by activating Nb CML19
  • +7
  • xianchao sun,
  • Shunyu Xiang,
  • Jing Wang,
  • Xiaoyan Wang,
  • Xiaozhou Ma,
  • Haoran Peng,
  • Xin Zhu,
  • Jin Huang,
  • Daibin Wang,
  • Lisong Ma
xianchao sun
Southwest University College of Plant Protection

Corresponding Author:sunxianchao@163.com

Author Profile
Shunyu Xiang
Southwest University College of Plant Protection
Author Profile
Jing Wang
Southwest University College of Plant Protection
Author Profile
Xiaoyan Wang
Southwest University College of Plant Protection
Author Profile
Xiaozhou Ma
Southwest University College of Plant Protection
Author Profile
Haoran Peng
Southwest University College of Plant Protection
Author Profile
Xin Zhu
Southwest University College of Plant Protection
Author Profile
Jin Huang
Southwest University
Author Profile
Daibin Wang
Southwest University
Author Profile
Lisong Ma
Hebei Agricultural University
Author Profile

Abstract

Control of plant virus diseases largely depends on the induced plant defense achieved by the external application of synthetic chemical inducers with the ability to modify defense-signaling pathways. However, most of the molecular mechanisms underlying these chemical inducers remain unknown. Here, we developed a lentinan-loaded hydrogel with a core-shell structure and discovered how it protects plants from different virus infections. The hydrogel was synthesized by adding a chitosan shell on the surface of the polyanion sodium alginate-Ca 2+-lentinan (LNT) hydrogel (SL-gel) to form a CSL-gel. CSL-gels exhibit the capacity to prolong the stable release of lentinan and promote Ca 2+ release. Application of CSL-gels on the root of plants induces broad-spectrum resistance against TMV, TRV, PVX and TuMV). RNA-seq analysis identified that the calmodulin-like protein 19 gene ( NbCML19) is upregulated by the sustained release of Ca 2+ from the CSL-gel, and silencing and overexpression of NbCML19 alter the susceptibility and resistance of tobacco to TMV. Our findings provide evidence that this novel and synthetic CSL-gel strongly inhibits the infection of plant viruses by the sustainable release of LNT and Ca 2+. This study uncovers a novel mode of action by which CSL-gels trigger NbCML19 expression through the stable and sustained release of Ca 2+.
25 Mar 2023Submitted to Plant, Cell & Environment
25 Mar 2023Submission Checks Completed
25 Mar 2023Assigned to Editor
25 Mar 2023Review(s) Completed, Editorial Evaluation Pending
05 Apr 2023Reviewer(s) Assigned
28 Apr 2023Editorial Decision: Revise Minor
25 Jun 20231st Revision Received
04 Jul 2023Submission Checks Completed
04 Jul 2023Assigned to Editor
11 Jul 2023Review(s) Completed, Editorial Evaluation Pending
13 Jul 2023Editorial Decision: Revise Minor
26 Jul 20232nd Revision Received
28 Jul 2023Submission Checks Completed
28 Jul 2023Assigned to Editor
28 Jul 2023Review(s) Completed, Editorial Evaluation Pending
29 Jul 2023Editorial Decision: Accept