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The interplay between autophagy and chloroplast vesiculation pathways under dark-induced senescence
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  • Wagner Araújo,
  • Jessica Barros,
  • João Cavalcanti,
  • Karla Pimentel,
  • Sahar Magen,
  • Yoram Soroka,
  • David Barbosa Medeiros,
  • Adriano Nunes-Nesi,
  • Alisdair R. Fernie,
  • Tamar Avin-Wittenberg
Wagner Araújo
Universidade Federal de Vicosa Departamento de Biologia Vegetal

Corresponding Author:wlaraujo@ufv.br

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Jessica Barros
Universidade Federal de Vicosa Departamento de Biologia Vegetal
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João Cavalcanti
Universidade Federal do Amazonas
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Karla Pimentel
Universidade Federal de Vicosa Departamento de Biologia Vegetal
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Sahar Magen
Hebrew University of Jerusalem Department of Plant and Environmental Sciences
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Yoram Soroka
Hebrew University of Jerusalem Department of Plant and Environmental Sciences
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David Barbosa Medeiros
Max-Planck-Institut fur molekulare Pflanzenphysiologie
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Adriano Nunes-Nesi
Universidade Federal de Vicosa Departamento de Biologia Vegetal
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Alisdair R. Fernie
Max-Planck-Institut fur molekulare Pflanzenphysiologie
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Tamar Avin-Wittenberg
Hebrew University of Jerusalem Department of Plant and Environmental Sciences
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Abstract

In cellular circumstances where carbohydrates are scarce, plants can use alternative substrates for cellular energetic maintenance. In plants, the main protein reserve is present in the chloroplast, which contains most of the total leaf proteins and represents a rich source of nitrogen and amino acids. Autophagy plays a key role in chloroplast breakdown, a well-recognized symptom of both natural and stress-induced plant senescence. Remarkably, an autophagic-independent route of chloroplast degradation associated with Chloroplast Vesiculation (CV) gene was recently demonstrated. During extended darkness, CV is highly induced in the absence of autophagy, contributing to the early senescence phenotype of atg mutants. To further investigate the role of CV under dark-induced senescence conditions, mutants with low expression of CV ( amircv) and double mutants amircv1xatg5 were characterized. Following darkness treatment, no aberrant phenotypes were observed in amircv single mutants; however, amircv1xatg5 double mutants displayed early senescence and enhanced dismantling of chloroplast and membrane structures under these conditions. Metabolic characterization revealed that the functional lack of both CV and autophagy leads to higher impairment of amino acid release and differential organic acid accumulation during starvation conditions. The data obtained are discussed in the context of the role of CV and autophagy, both in terms of cellular metabolism and the regulation of chloroplast degradation.
03 Mar 2023Submitted to Plant, Cell & Environment
03 Mar 2023Submission Checks Completed
03 Mar 2023Assigned to Editor
05 Mar 2023Review(s) Completed, Editorial Evaluation Pending
10 Mar 2023Reviewer(s) Assigned
14 Apr 2023Editorial Decision: Revise Minor
15 Jul 20231st Revision Received
15 Jul 2023Submission Checks Completed
15 Jul 2023Assigned to Editor
22 Jul 2023Review(s) Completed, Editorial Evaluation Pending
24 Jul 2023Reviewer(s) Assigned
15 Aug 2023Editorial Decision: Accept