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Decellularized periosteum promotes guided bone regeneration via manipulation of macrophage polarization
  • +4
  • Jiayang Li,
  • Dongming He,
  • Longwei Hu,
  • Siyi Li,
  • Chenping Zhang,
  • Xuelai Yin,
  • Zhen Zhang
Jiayang Li
Shanghai Stomatological Hospital
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Dongming He
Shanghai 9th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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Longwei Hu
Shanghai 9th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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Siyi Li
Shanghai 9th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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Chenping Zhang
Shanghai 9th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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Xuelai Yin
Shanghai 9th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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Zhen Zhang
Shanghai 9th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine

Corresponding Author:zz_nirvana@163.com

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Abstract

Periosteum has shown potential as an effective barrier membrane for guided bone regeneration (GBR). However, if recognized as a “foreign body”, insertion of a barrier membrane in GBR treatment will inevitably alter the local immune microenvironment and subsequently influence bone regeneration. The aim of this investigation was to fabricate decellularized periosteum (DP) and investigate its immunomodulatory properties in GBR. DP was successfully fabricated from periosteum from the mini-pig cranium. In vitro experiments indicated that the DP scaffold modulated macrophage polarization toward a pro-regenerative M2 phenotype, which in turn facilitated migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. A rat GBR model with a cranial critical-size defect was established, and our in vivo experiment confirmed the beneficial effects of DP on the local immune microenvironment and bone regeneration. Collectively, the findings of this study indicate that the prepared DP possesses immunomodulatory properties and represents a promising barrier membrane for GBR procedures.
26 Feb 2023Submitted to Biotechnology Journal
27 Feb 2023Submission Checks Completed
27 Feb 2023Assigned to Editor
09 Mar 2023Reviewer(s) Assigned
18 May 2023Review(s) Completed, Editorial Evaluation Pending
19 May 2023Editorial Decision: Revise Minor
01 Jun 20231st Revision Received
05 Jun 2023Submission Checks Completed
05 Jun 2023Assigned to Editor
05 Jun 2023Reviewer(s) Assigned
06 Jun 2023Review(s) Completed, Editorial Evaluation Pending
07 Jun 2023Editorial Decision: Accept