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A novel cubic-exp evaluation algorithm considering non-symmetrical axial response signals of confocal microscopes
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  • Sebastian Hagemeier,
  • Tobias Pahl,
  • Johannes Breidenbach,
  • Peter Lehmann
Sebastian Hagemeier
Universitat Kassel Fachbereich 16 Elektrotechnik/Informatik

Corresponding Author:sebastian.hagemeier@uni-kassel.de

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Tobias Pahl
Universitat Kassel Fachbereich 16 Elektrotechnik/Informatik
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Johannes Breidenbach
Universitat Kassel Fachbereich 16 Elektrotechnik/Informatik
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Peter Lehmann
Universitat Kassel Fachbereich 16 Elektrotechnik/Informatik
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Abstract

The depth discrimination in confocal microscopy is based on the digital analysis of depth response signals obtained by each camera pixel during measurement. Various signal processing algorithms are used for this purpose. The accuracy of these algorithms is inter alia restricted by the axial symmetry of the signals. However, in practice response signals are rather asymmetrical especially in case of measurement objects with critical surface structures such as edges or steep flanks. We present a novel signal processing algorithm based on an exponential function with a cubic argument to handle asymmetrical and also symmetrical depth response signals. Results obtained by this algorithm are compared to those of commonly used signal processing algorithms. It turns out that the novel algorithm is more robust, more accurate and exhibits a repeatability of a similar order compared to other algorithms.
15 Feb 2023Submitted to Microscopy Research and Technique
17 Feb 2023Submission Checks Completed
17 Feb 2023Assigned to Editor
03 Mar 2023Review(s) Completed, Editorial Evaluation Pending
03 Mar 2023Reviewer(s) Assigned
26 May 2023Editorial Decision: Revise Major
07 Jun 20231st Revision Received
13 Jun 2023Submission Checks Completed
13 Jun 2023Assigned to Editor
13 Jun 2023Review(s) Completed, Editorial Evaluation Pending
14 Jun 2023Reviewer(s) Assigned
14 Jun 2023Editorial Decision: Accept