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The ever increasing necessity of mass spectrometry in dissecting protein post-translational modifications catalyzed by bacterial effectors
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  • Xiaoyun Liu,
  • Jie Jin,
  • Yi Yuan,
  • Wei Xian,
  • Zhiheng Tang,
  • Jiaqi Fu
Xiaoyun Liu
Peking University School of Basic Medical Sciences

Corresponding Author:xiaoyun.liu@bjmu.edu.cn

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Jie Jin
Peking University School of Basic Medical Sciences
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Yi Yuan
Peking University School of Basic Medical Sciences
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Wei Xian
Peking University School of Basic Medical Sciences
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Zhiheng Tang
Peking University School of Basic Medical Sciences
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Jiaqi Fu
The First Hospital of Jilin University
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Abstract

Protein post-translational modifications (PTMs), such as ADP-ribosylation and phosphorylation, regulate multiple fundamental biological processes in cells. During bacterial infection, effector proteins are delivered into host cells through dedicated bacterial secretion systems and can modulate important cellular pathways by covalently modifying their host targets. These strategies enable intruding bacteria to subvert various host processes, thereby promoting their own survival and proliferation. Despite rapid expansion of our understanding of effector-mediated PTMs in host cells, analytical measurements of these molecular events still pose significant challenges in the study of host-pathogen interactions. Nevertheless, with major technical breakthroughs in the last two decades, mass spectrometry (MS) has evolved to be a valuable tool for detecting protein PTMs and mapping modification sites. Additionally, large-scale PTM profiling, facilitated by different enrichment strategies prior to MS analysis, allows high-throughput screening of host enzymatic substrates of bacterial effectors. In this review, we summarize the advances in the studies of two representative PTMs (i.e., ADP-ribosylation and phosphorylation) catalyzed by bacterial effectors during infection. Importantly, we will discuss the ever increasing role of MS in understanding these molecular events and how the latest MS-based tools can aid in future studies of this booming area of pathogenic bacteria-host interactions.
14 Feb 2023Submitted to Molecular Microbiology
15 Feb 2023Submission Checks Completed
15 Feb 2023Assigned to Editor
17 Feb 2023Reviewer(s) Assigned
17 Mar 2023Review(s) Completed, Editorial Evaluation Pending
26 Mar 2023Editorial Decision: Revise Minor
18 Apr 20231st Revision Received
21 Apr 2023Submission Checks Completed
21 Apr 2023Assigned to Editor
21 Apr 2023Review(s) Completed, Editorial Evaluation Pending
21 Apr 2023Editorial Decision: Accept