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Dual-slit confocal light-sheet microscopy using birefringent crystals
  • +2
  • Xin Xu,
  • Hone Ye,
  • Jixiang Wang,
  • Xin Zhang,
  • Guohua Shi
Xin Xu
University of Science and Technology of China

Corresponding Author:xux.stu@sibet.ac.cn

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Hone Ye
Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science
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Jixiang Wang
University of Science and Technology of China
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Xin Zhang
Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Science
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Guohua Shi
University of Science and Technology of China
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Abstract

We present a method to use a birefringent crystal for generating two illumination beams in a digital scanned laser light-sheet microscopy (DSLM) system. Upon this, a conventional confocal DSLM can be easily upgraded to a dual-slit confocal DSLM with two-fold imaging speed. We have implemented this method to our bidirectional DSLM system, locating two identical calcite crystals on both illumination paths from both sides of the sample. The neurons of in vivo larval zebrafish have been fast imaged with sterling image quality, especially ~2.5 times higher contrast, compared to the conventional DSLM.
15 Feb 2023Submitted to Journal of Biophotonics
15 Feb 2023Submission Checks Completed
15 Feb 2023Assigned to Editor
15 Feb 2023Review(s) Completed, Editorial Evaluation Pending
15 Feb 2023Reviewer(s) Assigned
08 Mar 2023Editorial Decision: Revise Major
31 Mar 20231st Revision Received
31 Mar 2023Submission Checks Completed
31 Mar 2023Assigned to Editor
31 Mar 2023Review(s) Completed, Editorial Evaluation Pending
31 Mar 2023Reviewer(s) Assigned
16 Apr 2023Editorial Decision: Accept