loading page

Multi-omics analysis reveals a link between Brassica -specific miR1885 and rapeseed tolerance to low temperature
  • +4
  • Xiang Yu,
  • Pengfei Xu,
  • Wenting Zhang,
  • Xuan Wang,
  • Yantao Zhu,
  • Wanqi Liang,
  • Yuke He
Xiang Yu
Shanghai Jiao Tong University School of Life Sciences and Biotechnology

Corresponding Author:yuxiang2021@sjtu.edu.cn

Author Profile
Pengfei Xu
Shanghai Jiao Tong University School of Life Sciences and Biotechnology
Author Profile
Wenting Zhang
Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences
Author Profile
Xuan Wang
Shanghai Normal University
Author Profile
Yantao Zhu
Hybrid Rapeseed Research Center of Shaanxi Province
Author Profile
Wanqi Liang
Shanghai Jiao Tong University School of Life Sciences and Biotechnology
Author Profile
Yuke He
Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences
Author Profile

Abstract

Brassica crops include various edible vegetable and plant oil crops, and their production is limited by low temperature beyond their tolerant capability. The key regulators of low-temperature resistance in Brassica remain largely unexplored. To identify post-transcriptional regulators of plant response to low temperature, we performed small RNA profiling, and found that 16 known miRNAs were responsive to cold treatment in Brassica rapa. The cold response of seven of those miRNAs were further confirmed by qRT-PCR and/or northern blotting analyses. In parallel, a genome-wide association study of 220 accessions of Brassica napus identified four candidate MIRNA genes, all of which were cold-responsive, at the loci associated with low temperature resistance. Specifically, these large-scale data analyses revealed a link between miR1885 and the plant response to low temperature in both B. rapa and B. napus. Using 5′ rapid amplification of cDNA ends approach, we validated that miR1885 can cleave its putative target transcripts, Bn.TIR.A09 and Bn.TNL.A03, in B. napus. Furthermore, overexpression of miR1885 in Semi-winter type B. napus decreased the mRNA abundance of Bn.TIR.A09 and Bn.TNL.A03, resulting in increased sensitivity to low temperature. Knocking down of miR1885 in Spring type B. napus led to increased mRNA abundance of its targets and improved rapeseed tolerance to low temperature. Together, our results suggested that the loci of miR1885 and its targets could be potential candidates for the molecular breeding of low temperature-tolerant Spring type Brassica crops.
28 Jan 2023Submitted to Plant, Cell & Environment
28 Jan 2023Submission Checks Completed
28 Jan 2023Assigned to Editor
28 Jan 2023Review(s) Completed, Editorial Evaluation Pending
09 Mar 2023Reviewer(s) Assigned
29 Apr 2023Editorial Decision: Revise Minor
25 Jun 20231st Revision Received
27 Jul 2023Submission Checks Completed
27 Jul 2023Assigned to Editor
29 Jul 2023Review(s) Completed, Editorial Evaluation Pending
01 Aug 2023Editorial Decision: Accept