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Auxotrophs compromise bio-production in yeast Saccharomyces cerevisiae
  • +3
  • Chunxiao Yan,
  • Ning Gao,
  • Xuan Cao,
  • Lun Yao,
  • Yongjin Zhou,
  • Jiaoqi Gao
Chunxiao Yan
Chinese Academy of Sciences Dalian Institute of Chemical Physics

Corresponding Author:ycx18335464063@163.com

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Ning Gao
Chinese Academy of Sciences Dalian Institute of Chemical Physics
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Xuan Cao
Chinese Academy of Sciences Dalian Institute of Chemical Physics
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Lun Yao
Chinese Academy of Sciences Dalian Institute of Chemical Physics
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Yongjin Zhou
Dalian Institute of Chemical Physics
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Jiaoqi Gao
Dalian Institute of Chemical Physics Department of Biotechnology
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Abstract

Auxotrophic marker genes have widely used for genetic engineering in yeast. However, the effects of auxotrophic strains that are deficient in synthesis of amino acids or nucleotides on the growth and production are rarely reported. In this study, a total of eight auxotrophic strains with single knockout of selective markers were obtained to evaluate cell growth and free fatty acid (FFA) production in Saccharomyces cerevisiae with supplementing different concentrations of amino acids or nucleotides. Generally, except for gene ADE2, most auxotrophic strains possessed decreased cell growth and FFA production, which could be remedied by the higher concentrations of supplements. leu2Δ damaged both growth and production even with supplementation of 1000 mg/L leucine. Therefore, this study shows that auxotrophs compromise the metabolic engineering endeavor and provides a guidance in supplementing amino acids or nucleotides during fermentations for maximizing bio-productions.
10 Oct 2022Submitted to Biotechnology Journal
11 Oct 2022Submission Checks Completed
11 Oct 2022Assigned to Editor
12 Oct 2022Reviewer(s) Assigned
03 Nov 2022Review(s) Completed, Editorial Evaluation Pending
11 Nov 2022Editorial Decision: Revise Major
07 Dec 20221st Revision Received
07 Dec 2022Submission Checks Completed
07 Dec 2022Assigned to Editor
07 Dec 2022Reviewer(s) Assigned
20 Dec 2022Review(s) Completed, Editorial Evaluation Pending
19 Jan 2023Editorial Decision: Accept