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Enhanced β-Glucosidase in western flower thrips affects its interaction with the redox-based strategies of kidney beans under elevated CO 2
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  • Xiaowei Liu,
  • Yanhui Wang,
  • Hui Liu,
  • Xinyi Huang,
  • Lei Qian,
  • Baoqing Yang,
  • Yujing Xu,
  • Fajun Chen
Xiaowei Liu
Nanjing Agricultural University College of Plant Protection

Corresponding Author:2019202049@njau.edu.cn

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Yanhui Wang
Nanjing Agricultural University College of Plant Protection
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Hui Liu
Nanjing Agricultural University College of Plant Protection
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Xinyi Huang
Nanjing Agricultural University College of Plant Protection
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Lei Qian
Jiangsu Academy of Agricultural Sciences
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Baoqing Yang
Nanjing University of Chinese Medicine
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Yujing Xu
Nanjing University of Chinese Medicine
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Fajun Chen
Nanjing Agricultural University College of Plant Protection
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Abstract

β-Glucosidase is validated as an elicitor for early immune responses in plants and it was detected in the salivary glands of Frankliniella occidentalis in previous research. Seven differentially expressed genes encoding β-Glucosidase were obtained by comparing the transcriptomes of F. occidentalis adults grown under two different CO 2 concentrations (800 ppm vs. 400 ppm), which might be associated with the differences in the interaction between F. occidentalis adults and its host plant, Phaseolus vulgaris under different CO 2 levels. To verify this speculation, changes in defense responses based on the production and elimination of reactive oxygen species (ROS) in P. vulgaris leaves treated with three levels of β-Glucosidase activity under ambient CO 2 (aCO 2) and elevated CO 2 (eCO 2) were measured in this study. The results showed that both leaves infested with thrips and those sprayed with the pure β-Glucosidase solution showed significant increases in ROS levels under aCO 2 and eCO 2, and the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were increased correspondingly, while in leaves infested with FoβGlu-1-silenced thrips, the ROS levels and activities of these enzymes did not change significantly during the first 12 hours of injury regardless of CO 2 level. Besides, significantly higher levels of ROS and lower activities of SOD, POD and CAT in injured leaves under eCO 2 compared to aCO 2 were noticed, which would negatively affect P. vulgaris leaves and facilitate thrips damage.
10 Oct 2022Submitted to Plant, Cell & Environment
10 Oct 2022Submission Checks Completed
10 Oct 2022Assigned to Editor
16 Oct 2022Review(s) Completed, Editorial Evaluation Pending
17 Oct 2022Reviewer(s) Assigned
08 Dec 2022Editorial Decision: Revise Minor
22 Dec 20221st Revision Received
27 Dec 2022Submission Checks Completed
27 Dec 2022Assigned to Editor
31 Dec 2022Review(s) Completed, Editorial Evaluation Pending
03 Jan 2023Editorial Decision: Accept
09 Jan 2023Published in Plant, Cell & Environment. 10.1111/pce.14534