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Ca2+ Deficiency Triggers Panicle Degeneration in Rice Mediated by Ca2+/H+ Exchanger OsCAX1a
  • +9
  • Quan Gan,
  • Fengshun Song,
  • Chuanzhong Zhang,
  • Zhongmin Han,
  • Bin Teng,
  • Cuixiang Lin,
  • Dongfang Gu,
  • Jiajia Wang,
  • Huan Pei,
  • Ji Wu,
  • Jun Fang,
  • Dahu Ni
Quan Gan
Anhui Academy of Agricultural Sciences
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Fengshun Song
Anhui Academy of Agricultural Sciences
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Chuanzhong Zhang
Northeast Institute of Geography and Agroecology Chinese Academy of Sciences
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Zhongmin Han
Northeast Institute of Geography and Agroecology Chinese Academy of Sciences
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Bin Teng
Anhui Academy of Agricultural Sciences
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Cuixiang Lin
Anhui Academy of Agricultural Sciences
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Dongfang Gu
Anhui Academy of Agricultural Sciences
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Jiajia Wang
Anhui Academy of Agricultural Sciences
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Huan Pei
Anhui Academy of Agricultural Sciences
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Ji Wu
Anhui Academy of Agricultural Sciences
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Jun Fang
Northeast Institute of Geography and Agroecology Chinese Academy of Sciences
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Dahu Ni
Anhui Academy of Agricultural Sciences

Corresponding Author:dahuni1974@163.com

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Abstract

Increasing rice yield has always been one of the primary objectives of rice breeding. However, panicle degeneration, a complex phenomenon regulated by many genetic and environmental factors, often occurs in rice-growing regions and severely curbs rice yield. In this study, we obtained a new apical panicle degeneration mutant named ym48, which induces a marked degeneration rate and diminishes the final grain yield. Cellular and physiological analyses revealed that the apical panicle in ym48 undergoes programmed cell death, accompanied by excessive accumulations of peroxides. Following, the panicle degeneration gene OsCAX1a was identified, which was involved in Ca2+ transport in the ym48 mutant. In OsCAX1a, a relative conserved T to A substitution was noted at the 64th amino acid, which disrupted Ca2+ transport. Hydroponics assays and Ca2+ quantification confirmed that Ca2+ transport and distribution to apical tissues were restricted and over-accumulated in mutant sheath. Ca2+ transport between cytoplasm and vacuole was affected, and the reduced content of Ca2+ in vacuole and cell wall and the decreased of Ca2+ absorption were appeared in ym48 mutant. RNA-Seq data indicated that the abnormal CBL (calcineurin b-like proteins) pathway mediated by deficient Ca2+ might occur in mutant, resulting in the burst of ROS and programmed cell death in panicles. Our results explained the key role of OsCAX1a in Ca2+ transport and distribution and laid a foundation to further explore the genetic and molecular mechanisms of panicle degeneration and the efficiency of Ca2+ fertilization in rice.
08 Dec 2022Submitted to Plant, Cell & Environment
08 Dec 2022Submission Checks Completed
08 Dec 2022Assigned to Editor
08 Dec 2022Review(s) Completed, Editorial Evaluation Pending
13 Dec 2022Reviewer(s) Assigned
29 Dec 2022Editorial Decision: Revise Minor
18 Jan 20231st Revision Received
18 Jan 2023Submission Checks Completed
18 Jan 2023Assigned to Editor
19 Jan 2023Review(s) Completed, Editorial Evaluation Pending
20 Jan 2023Editorial Decision: Revise Minor
20 Jan 20232nd Revision Received
21 Jan 2023Submission Checks Completed
21 Jan 2023Assigned to Editor
21 Jan 2023Review(s) Completed, Editorial Evaluation Pending
21 Jan 2023Editorial Decision: Accept