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Exploring the phycosphere of Emiliania huxleyi: from bloom dynamics to microbiome assembly experiments
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  • Mariana Câmara dos Reis,
  • Sarah Romac,
  • Florence Le Gall,
  • Dominique Marie,
  • Miguel José Frada,
  • Gil Koplovitz,
  • Thierry Cariou,
  • Nicolas Henry,
  • Colomban de Vargas,
  • Christian Jeanthon
Mariana Câmara dos Reis
Station Biologique de Roscoff

Corresponding Author:maricamarareis@gmail.com

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Sarah Romac
Station Biologique de Roscoff
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Florence Le Gall
Station Biologique de Roscoff
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Dominique Marie
Station Biologique de Roscoff
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Miguel José Frada
Interuniversity Institute for Marine Sciences in Eilat
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Gil Koplovitz
Interuniversity Institute for Marine Sciences in Eilat
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Thierry Cariou
Station Biologique de Roscoff
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Nicolas Henry
Station Biologique de Roscoff
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Colomban de Vargas
Station Biologique de Roscoff
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Christian Jeanthon
Station Biologique de Roscoff
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Abstract

Coccolithophores have global ecological and biogeochemical significance as the most important calcifying marine phytoplankton group. The structure and selection of prokaryotic communities associated with the most abundant coccolithophore and bloom-forming species, Emiliania huxleyi, are still poorly known. In this study, we assessed the diversity of bacterial communities associated with an E. huxleyi bloom in the Celtic Sea, exposed axenic E. huxleyi cultures to prokaryotic communities derived from bloom and non-bloom conditions and followed the dynamics of their microbiome composition over one year. Bloom-associated prokaryotic communities were dominated by SAR11, Marine group II Euryarchaeota, Rhodobacterales and contained substantial proportions of known indicators of phytoplankton bloom demises such as Flavobacteriaceae and Pseudoalteromonadaceae. Taxonomic richness of replicated co-cultures resulting from natural communities with axenic E. huxleyi rapidly shifted and then stabilized over time, presumably by ecological selection favoring more beneficial populations. Recruited microbiomes from the environment were consistently dependent on the composition of the initial bacterioplankton community. Phycosphere-associated communities derived from the E. huxleyi bloom depth were highly similar to one another, suggesting deterministic processes, whereas cultures from non-bloom conditions show an effect of both deterministic processes and stochasticity. Overall, this work sheds new light on the importance of the initial inoculum composition in microbiome recruitment and elucidates the temporal dynamics of its composition and long-term stability.
14 Feb 2022Submitted to Molecular Ecology
16 Feb 2022Submission Checks Completed
16 Feb 2022Assigned to Editor
21 Feb 2022Reviewer(s) Assigned
21 Jun 2022Review(s) Completed, Editorial Evaluation Pending
02 Aug 2022Editorial Decision: Revise Minor
11 Oct 2022Review(s) Completed, Editorial Evaluation Pending
11 Oct 20221st Revision Received
16 Dec 2022Editorial Decision: Accept