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Media engineering in marine diatom Phaeodactylum tricornutum employing cost-effective substrates for sustainable production of high value renewables
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  • Mohammed Rehmanji,
  • Asha Nesamma,
  • Nida Khan,
  • Tasneem Fatma,
  • Pannaga Jutur
Mohammed Rehmanji
International Centre for Genetic Engineering and Biotechnology New Delhi

Corresponding Author:mohammedrehmanji@gmail.com

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Asha Nesamma
International Centre for Genetic Engineering and Biotechnology New Delhi
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Nida Khan
Jamia Millia Islamia Central University
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Tasneem Fatma
Jamia Millia Islamia Central University
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Pannaga Jutur
International Centre for Genetic Engineering and Biotechnology New Delhi
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Abstract

Phaeodactylum tricornutum is a marine diatom, and well-studied model of unicellular microalga. This diatom contains a wide range of high-value renewables (HVRs) with high commercial relevance owing to their importance in human nutrition and health. In this study, we screened P. tricornutum for biomass, eicosapentaenoic acid (EPA) and fucoxanthin production under photoautotrophic and mixotrophic condition with various substrate combinations. Results highlights that culture supplemented with glycerol and urea lead to enhanced biomass, biochemical and HVR production. Further continuous feeding of urea in glycerol supplemented medium results in an increase in biomass yield (0.77 g L-1) by ~ 2-fold. Additionally, continuous feeding of urea channelizes the carbon flux towards biosynthesis of fatty acids increasing FAME content by ~2-fold as compared to the control conditions. Overall EPA and fucoxanthin production was 27 mg L-1 and 11 mg L-1 (~2 & 4 fold) in urea fed cultures respectively. Present study demonstrates efficient valorization of cost-effective substrates such as glycerol and urea for the production of high-value renewables in P. tricornutum.
19 Dec 2021Submitted to Biotechnology Journal
20 Dec 2021Submission Checks Completed
20 Dec 2021Assigned to Editor
03 Jan 2022Reviewer(s) Assigned
11 Feb 2022Editorial Decision: Revise Major
18 Apr 20221st Revision Received
20 Apr 2022Submission Checks Completed
20 Apr 2022Assigned to Editor
26 Apr 2022Reviewer(s) Assigned
31 May 2022Editorial Decision: Accept
Oct 2022Published in Biotechnology Journal volume 17 issue 10 on pages 2100684. 10.1002/biot.202100684