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Non-contact three-dimensional cell cluster formation on demand in open dishware using focused surface acoustic waves through a couplant layer
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  • Jiyang Mei,
  • Aditya Vasan,
  • Uri Magaram,
  • Kenjiro Takemura,
  • Sreekanth Chalasani,
  • James Friend
Jiyang Mei
University of California San Diego
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Aditya Vasan
University of California San Diego
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Uri Magaram
Salk Institute for Biological Studies
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Kenjiro Takemura
Keio Gijuku Daigaku
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Sreekanth Chalasani
Salk Institute for Biological Studies
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James Friend
University of California San Diego
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Abstract

Three-dimensional cell agglomerates are broadly useful in tissue engineering and drug testing. We report a well-free method to form large (1-mm) multicellular clusters using 100-MHz surface acoustic waves (SAW) without direct contact with the media or cells. A fluid couplant is used to transformthe SAW into acoustic streaming in the cell-laden media held in a petri dish. The couplant transmits longitudinal sound waves, forming a Lamb wave in the petri dish that, in turn, produces longitudinal sound in the media. Due to recirculation, human embryonic kidney (HEK293) cells in the dish are carried to the center of the coupling location, forming a cluster in less than 10 min. A few minutes later, these clusters may then be translated and merged to form large agglomerations, and even repeatedly folded to produce a roughly spherical shape of over 1 mm in diameter for incubation—without damaging the existing intercellular bonds. Calcium ion signaling through these clusters and confocal images of multiprotein junctional complexes suggest a continuous tissue construct: intercellular communication. They may be formed at will, and the method is feasibly useful for formation of numerous agglomerates in a single petri dish.