Objectives: Clinical biobank is an important way to collect biological samples of various diseases, which is of great significance for clinical research. However, the relationship of clinical biobank and clinical disciplines still need to be further explored, thus we conducted a survey among medical and scientific professionals in a hospital of China. Method: The survey was conducted online, and electronic questionnaires were used to investigate knowledge, utilization and demand of biobank of medical and scientific professionals in different clinical disciplines. In terms of data analysis, chi-square test and rank sum test were used to analyze the collected data. Results: A total of 182 valid questionnaires were collected. The overall survey participation rate of key disciplines is higher than that of non-key disciplines. Key disciplines have higher score on the knowledge of biobank than non-key disciplines. The proportion of sample storage and sample delivery of key disciplines are far higher than non-key disciplines, and key disciplines have higher sample delivery rate than non-key disciplines. The proportion of people who are willing to pay to store samples and people who are willing to pay more than 5000 yuan per year for sample storage in key disciplines are higher than that in non-key disciplines. The per capita national grant application, the approval rate of national grant and per capita published English papers of key disciplines are higher than those of non-key disciplines. Conclusion: Key disciplines are more enthusiastic about the construction of biobank. On the whole, the knowledge, utilization and demand on biobank of key disciplines are better than those of non-key disciplines. The development of key disciplines is better than that of non-key disciplines. The development of clinical biobank is closely related to the development of clinical disciplines, and clinical biobank is an important booster for the development of clinical disciplines.