Interpreting seismo-acoustic signals is critical for assessing and characterizing changes in volcanic vents and has implications for interpreting volcanic unrest. This is especially relevant for Stromboli volcano (Italy), an active stratovolcano with a complex plumbing system, continuous activity, and recurring paroxysms. Stromboli is known for its consistent Strombolian style of eruption, multiple active vents on its crater terrace, and for occasional structural modifications including explosive excavation and/or collapsing craters due to near-surface changes to the plumbing system. This study addresses a single localized collapse of the crater terrace, occurring in May of 2019, when one of Stromboli’s vents changed from a pronounced hornito to a pit crater, resulting in a shift in eruption style at this vent from jetting to Strombolian. The days before and after this transition were recorded with eight infrasound sensors and three seismic geophones located on the crater terrace. We investigate the seismo-acoustic timing of these signals as well as the ratio between seismic and acoustic energy to identify changes associated with eruptive signals and associated variations in location of the eruptive sources. This work highlights the effectiveness of seismo-acoustic data analysis, provides insight into Stromboli’s structural modifications, and builds a foundation for focused analysis of seismo-acoustic signals associated with Stromboli and other open-vent volcanic systems.