Thunderstorms exhibiting anomalous charge structures (ACSs, i.e., anomalous storms) are comprised by more riming ice carrying net positive versus negative charge, thought to result from increased mixed-phase cloud liquid water content (LWC). Anomalous storms are rarely observed in the United States (US) outside of the Great Plains (GP) region, where environmental conditions that suppress warm precipitation efficiency and support robust updrafts are thought to favor increased mixed-phase LWC. Two rare anomalous supercells in the Southeastern (SE) US exhibited similar charge structure characteristics as observed in GP anomalous storms, including a deep positive (negative) charge layer associated with riming (non-riming) ice in the lower (upper) mixed-phase region. However, most characteristics associated with SE anomalous environments were not consistent with those in the GP. A more rigorous evaluation of hypotheses concerning ACS development and their observation in the SE compared electrical, kinematic, microphysical, and environmental properties between the two anomalous and two SE normal supercells (i.e., exhibiting normal charge structures). Similar metrics of warm precipitation efficiency were observed in each. However, lower relative humidity in the charging region of the SE anomalous storms uniquely matched environmental characteristics in GP anomalous storms and differentiated SE anomalous from normal environments, suggesting the relative importance of saturation ratio alongside LWC in positive charging of riming ice. Differences were also observed in charge region characteristics and flash locations between a SE anomalous and normal storm. As riming ice increased in the negative charge region of the ACS, flash initiation locations were increasingly observed in stronger updrafts and updraft gradients compared with the normal storm. The evolution of microphysical characteristics of the negative charge region in the anomalous storm suggested an increase in normal alongside anomalous charging, indicating that variability in charging polarity may impact spatial flash relationships with the updraft. Further work is needed to diagnose whether particle-scale charging variability influences flash rate relationships with convective parameters such as updraft or graupel volume.